Quote Originally Posted by LEFTLANE
I was just seeing if anyone has got pups off two males in same litter. I've herd that most people get pups off one sire when trying this. We are going to dna profile this litter. Im just wanted ti know if anyone else had a situation like this before and it worked out for them.
Some people say that the sperm will "fight" each other if a bitch is bred to two males for a dual sired litter. I don't know whether or not that is true, but I do know that it must not matter much one way or the other, as I've seen PLENTY of litters that were sired by two or more fathers in clinics over the years. I don't think most of the people who've tried this have actually DONE the DNA tested necessary to prove which father is which in such a scenario - I think many look at the pups that are produced and make an "educated guess" as to which father it might've been and deduce the likelihood of all of the puppies being off of him based on uniformity of the litter -- like when you hear "yeah, we bred her to a black nose and a red nose, all the pups were this colored nose, so they have to be off of this dog" and depending on the momma and her genes, that can be a total misnomer in most cases, especially given the discrepancy between colorations and how many people actually know differences between red/chocolate etc. and so forth.

In short, yes, it's definitely possible, and you'll definitely need a DNA test to be sure.